Generator
Part:BBa_K2201331:Design
Designed by: Laura Schlueter Group: iGEM17_Bielefeld-CeBiTec (2017-10-20)
NM-domain of Sup35 with an amber codon at position 21 under T7-promoter control
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The CTA codon were inserted via site directed mutagenesis and the T7 promoter via bio brick assembly.
Source
BBa_K2201231